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Santa Cruz Biotechnology western blotting imagequant las 4000 anti tsg101 antibody
(A) SDS-PAGE analysis of gradient fractions. Fractions 1 to 12 (90 μL) were loaded. (B) Western Blot analysis of gradient fractions for the exosome marker proteins Alix and <t>Tsg101.</t> Exosome proteins were observed in fractions 6 to 7.
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Image Search Results


(A) SDS-PAGE analysis of gradient fractions. Fractions 1 to 12 (90 μL) were loaded. (B) Western Blot analysis of gradient fractions for the exosome marker proteins Alix and Tsg101. Exosome proteins were observed in fractions 6 to 7.

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Cushioned–Density Gradient Ultracentrifugation (C–DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes

doi: 10.1007/978-1-4939-7652-2_7

Figure Lengend Snippet: (A) SDS-PAGE analysis of gradient fractions. Fractions 1 to 12 (90 μL) were loaded. (B) Western Blot analysis of gradient fractions for the exosome marker proteins Alix and Tsg101. Exosome proteins were observed in fractions 6 to 7.

Article Snippet: SDS-PAGE and western blotting ImageQuant LAS 4000 Anti-TSG101 antibody (Santa Cruz Biotechnology, sc-7964) Anti-Alix antibody (Cell Signaling Technology, 2171) IRDye 680RD goat anti-mouse IgG (LI-COR) IRDye 800CW goat anti-rabbit IgG (LI-COR) Odyssey infrared imaging system 2.5.

Techniques: SDS Page, Western Blot, Marker